National Gnotobiotic Rodent Resource Center (NGRRC)

Overview of Services

The National Gnotobiotic Rodent Resource Center (NGRRC) is a nationwide resource for germ-free and gnotobiotic mice and associated services. Gnotobiotic means “known life” and refers to the selective colonization of germ-free mice with microbes. The Center is an expansion of the Gnotobiotic Core of the Center for Gastrointestinal Biology and Disease (CGIBD), an NIH-NIDDK funded Digestive Diseases Research Core Center at UNC-Chapel Hill and North Carolina State University. Equipment for the expansion of the CGIBD Gnotobiotic Core was provided initially by a North Carolina Biotechnology Center grant with additional funds from the UNC-CH School of Medicine and from an administrative supplement to the CGIBD. In 2004, we leveraged our expertise in gnotobiotics to obtain a grant from the NIH’s National Center for Research Resources, Comparative Medicine Group to establish the NGRRC. Current funding for the NGRRC is provided primarily by the NIH Office of the Director (P40 OD010995), with additional funds from the CGIBD’s NIDDK grant (P30 DK034987) and the Crohn’s and Colitis Foundation.

Tissue/fluid/stool collection and shipping
Germ-free mouse shipping
Germ-free mouse colonization
Germ-free mouse procedures (e.g. injections, blood collection, oral gavage, etc.)
Minor germ-free mouse surgery
Test custom diets for sterility
Gnotobiotic experiments in NGRRC isolators
New germ-free derivations
On-site gnotobiotic training course



R. Balfour Sartor MD/Director 919-966-0148
Allison Rogala DVM, DACLAM/Co-Director 919-966-2901 
Josh Frost BA, RLATg/Manager 919-843-8836
Amy Smither MBA/Adminstrator, CGIBD 919-966-8381 

Location and hours of operation

Hours Location

Monday - Friday
7AM - 3:30PM

120 Mason Farm Rd
Chapel Hill, NC 27516

Links and Resources

  1. NGRRC
  2. NGRRC Request/Inquiry Form
  3. NGRRC New Project Request
  4. New Germ-Free Mouse Derivation Request
  5. Request to Schedule Onsite Training in Gnotobiotic Techniques at UNC


Name Role Phone Email Location
Josh Frost
Genetic Medicine