ALERT

The MLI Tissue Procurement and Cell Culture Core will be closed for the holidays starting December 26-January 2, 2023. All media/cell orders will need to be placed by December 15th, 2022. Our last shipping date will be December 20th, 2022. We will resume shipping January 9th, 2023.

 

The Pathology Services Core (PSC) will be closed from December 26th to January 2nd. Please do not submit any samples during this time as sample pickup will not be available. All submissions should be completed by December 22, 2022. Standard operations resume on January 3, 2023.

 

Thank you for your support, and we hope you have a wonderful Holiday Season!

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MHI Cardiovascular Physiology and Phenotyping Core (CPPC)

Mission:

The MHI Cardiovascular Physiology and Phenotyping Core (CPPC) provides surgical, imaging and functional assessment services with a focus on cardiovascular system in rodents. 

Services (Please contact the Core before the first order): 

1. Animal models and microsurgery*

  • Myocardial Infarction (LCA permanent ligation, verified by ECG measurement)**
  • Ischemia-Reperfusion (LCA temporary ligation, verified by ECG measurement)**
  • Pressure Overload (Aortic constriction, verified by PW Doppler ultrasound)**
  • Minipump/Telemetry Implantation in mice and rats
  • Arterial and venous cannulation
  • Carotid or renal artery ligation
  • ferric chloride-induced thrombosis model
  • Cardiac arrest/resuscitation murine model
  • Customized surgeries 

* Mice are discounted the full price if
    a. They do not make it off the surgical table or
    b. Technical failure: the blood flow velocity in TAC mice or ECG signal in MI mice does not match the requirement.
** The PW Doppler measurement and ECG measurement are included in services. Data are available upon reques.

2. Echocardiographic and electrocardiographic studies

  • B/M mode (parasternal long or short axis view of the left ventricle) in conscious mice
  • Color Doppler and Pulsed Wave Doppler (Apical four chamber view or blood flow velocity)
  • ECG measurement

3. Isolation of adult and neonatal cardiomyocytes

  • Langendorff-perfusion based isolation of adult cardiomyocytes

4. Functional assessments of cardiovascular phenotypes

  • PV loops
  • Langendorff and working heart system
  • Invasive blood pressure measurement

5. Equipment rent

  • Vevo2100 Ultrasound system (Echocardiography)
  • CODA® High Throughput System (Non-invasive blood pressure measurement)
  • Surgical table with Zeiss two-head surgical Microscope
  • Ultra Precise Small Animal Stereotaxic instrument
  • Workstation with LabChart 8.0 pro and Vevo Lab

Leadership

Haifeng Yin Core Director
Jonathan C. Schisler Faculty Director

Location and hours of operation

Hours Location

Monday - Friday 

9AM - 5PM

111 Mason farm Road

3306 MBRB

Chapel Hill, NC 27599

Links and Resources

https://www.med.unc.edu/mhi/surgicalmodels/

Contacts

Name Role Phone Email Location
Haifeng Yin
Core Director
 
919-889-7988
 
cardiocore@unc.edu
 
3312A MBRB
 

Service list


Search available services: View: by category alphabetically
Assessment of cardiac function (4)
Isolation of Cardiomyocytes (1)
Microsurgery - Mouse (6)
Microsurgery - Rat (1)