MHI Cardiovascular Physiology and Phenotyping Core (CPPC)

Mission:

The MHI Cardiovascular Physiology and Phenotyping Core (CPPC) provides surgical, imaging and functional assessment services with a focus on cardiovascular system in rodents.

Services (Please contact the Core before the first order):

1. Animal models and microsurgery*

Myocardial Infarction (LCA permanent ligation, verified by ECG measurement)**
Ischemia-Reperfusion (LCA temporary ligation, verified by ECG measurement)**
Pressure Overload (Aortic constriction, verified by PW Doppler ultrasound)**
Minipump/Telemetry Implantation in mice and rats
Arterial and venous cannulation
Carotid or renal artery ligation
ferric chloride-induced thrombosis model
Cardiac arrest/resuscitation murine model
Customized surgeries

* Mice are discounted the full price if
a. They do not make it off the surgical table or
b. Technical failure: the blood flow velocity in TAC mice or ECG signal in MI mice does not match the requirement.
** The PW Doppler measurement and ECG measurement are included in services. Data are available upon reques.

2. Echocardiographic and electrocardiographic studies

B/M mode (parasternal long or short axis view of the left ventricle) in conscious mice
Color Doppler and Pulsed Wave Doppler (Apical four chamber view or blood flow velocity)
ECG measurement

3. Isolation of adult and neonatal cardiomyocytes

Langendorff-perfusion based isolation of adult cardiomyocytes

4. Functional assessments of cardiovascular phenotypes

PV loops
Langendorff and working heart system
Invasive blood pressure measurement

5. Equipment rent

Vevo2100 Ultrasound system (Echocardiography)
CODA® High Throughput System (Non-invasive blood pressure measurement)
Surgical table with Zeiss two-head surgical Microscope
Ultra Precise Small Animal Stereotaxic instrument
Workstation with LabChart 8.0 pro and Vevo Lab

Leadership

Jonathan C. Schisler	Faculty Director
Charlene M. Santos	Core Director
Aung Moe Zaw	Core Manager
Delphine Bull Christman	Billing Manager

Location and hours of operation

Hours

Location

Monday - Friday

9AM - 5PM

111 Mason farm Road

3306 MBRB

Chapel Hill, NC 27599

Links and Resources

https://www.med.unc.edu/mhi/surgicalmodels/

Contacts

Name	Role	Phone	Email	Location
Aung Moe Zaw	Core Manager	(919) 966-3036	aung-moe_zaw@med.unc.edu	3006A MBRB
Charlene M. Santos	Core Director	(919) 843-7988	csantos@unc.edu
Jonathan C. Schisler	Faculty Director	(919) 843-8708	schisler@unc.edu	3340C MBRB
Delphine Bull Christman	Business Officer	(919) 962-4559	delphine_bull@med.unc.edu	3356 Genome Science Bldg

Service list

Name	Description	Price
Alzet Osmotic Pump Implantation	Surgical implantation of an Isoproterenol Mini-Pump to Induce cardiac hypertrophy and heart failure	Inquire
ECG measurement	Mouse ECG measurement.	Inquire
Echo - B/M mode	Ultrasound images of the left ventricle in the long axis, short asix or four chamber view.	Inquire
Echo - Doppler	1. Color and pulsed wave Doppler to measure blood flow direction and flow velocity. 2. Mitral valve Doppler in apical four chamber view to assess E′, A′, and S waves.	Inquire
Ferric Chloride Carotid Artery Thrombosis Assay	FeCl3 Induced Carotid Arterial Injury Thrombosis Model. The blood flow will be monitored using the Transonic Flowmeter.	Inquire
Ischemia-Reperfusion (I-R) - 45minsPer sample	Temporary ligation of left coronary artery for 30- 45 mins to induce Ischemia/Reperfusion. ECG measurement is used to monitor Ischemia and Reperfusion.	Inquire
Mouse Adult Cardiomyocytes	Isolation of mouse adult cardiomyocytes using the Langendorff-based method.	Inquire
Myocardial Infarction (MI)	Permanent ligation of left coronary artery to induce myocardia infarction.	Inquire
PV loops or Right or Left ventricular pressure measurements or Blood pressure measurement		Inquire
Telemetry Implantation	Surgical implantation of telemetry devices for measuring physiologic signals such as pressure, biopotential, activity and temperature.	Inquire
Telemetry Implantation		Inquire
Transverse aortic constriction (TAC)	Partially aortic binding to induce in LV hypertrophy. The severity of TAC is determined by Doppler ultrasound measurements.	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Assessment of cardiac function (4)
ECG measurement	Mouse ECG measurement.	Inquire
Echo - B/M mode	Ultrasound images of the left ventricle in the long axis, short asix or four chamber view.	Inquire
Echo - Doppler	1. Color and pulsed wave Doppler to measure blood flow direction and flow velocity. 2. Mitral valve Doppler in apical four chamber view to assess E′, A′, and S waves.	Inquire
PV loops or Right or Left ventricular pressure measurements or Blood pressure measurement		Inquire
▼ ► Isolation of Cardiomyocytes (1)
Name	Description	Price
Mouse Adult Cardiomyocytes	Isolation of mouse adult cardiomyocytes using the Langendorff-based method.	Inquire
▼ ► Microsurgery - Mouse (6)
Name	Description	Price
Alzet Osmotic Pump Implantation	Surgical implantation of an Isoproterenol Mini-Pump to Induce cardiac hypertrophy and heart failure	Inquire
Ferric Chloride Carotid Artery Thrombosis Assay	FeCl3 Induced Carotid Arterial Injury Thrombosis Model. The blood flow will be monitored using the Transonic Flowmeter.	Inquire
Ischemia-Reperfusion (I-R) - 45minsPer sample	Temporary ligation of left coronary artery for 30- 45 mins to induce Ischemia/Reperfusion. ECG measurement is used to monitor Ischemia and Reperfusion.	Inquire
Myocardial Infarction (MI)	Permanent ligation of left coronary artery to induce myocardia infarction.	Inquire
Telemetry Implantation	Surgical implantation of telemetry devices for measuring physiologic signals such as pressure, biopotential, activity and temperature.	Inquire
Transverse aortic constriction (TAC)	Partially aortic binding to induce in LV hypertrophy. The severity of TAC is determined by Doppler ultrasound measurements.	Inquire
▼ ► Microsurgery - Rat (1)
Name	Description	Price
Telemetry Implantation		Inquire