LCCC Translational Genomics Lab (TGL)

Description

The UNC Lineberger Comprehensive Cancer Center (LCCC) Translational Genomics Lab (TGL) is a high-throughput core facility established in 2011 to provide comprehensive genomic services for investigators. Located in Marsico Hall on UNC’s medical campus, TGL has 1,500 square feet of laboratory space and 200 square feet of office space. TGL’s mission is to support translational human cancer genomics research at UNC by providing a resource that balances rigor and flexibility to meet the needs of academic and clinical research that can ultimately produce optimized and validated processes for adoption in clinical labs.

Services

TGL performs sample processing and analyses for the molecular, pathologic, and genomic characterization of patient-derived specimens in preclinical research projects and clinical protocols. Our laboratory employs highly automated, as well as manual, platforms for reproducible high-throughput nucleic acid extraction, library preparation, and next-generation sequencing of tissue, cells, and blood samples. We have extensive experience generating usable data from highly degraded FFPE samples that would otherwise be rejected as unusable by larger sequencing facilities.

Service offerings include: DNA and RNA extraction from FFPE and snap frozen tissues, cells, and plasma; nucleic acid QC (Qubit, TapeStation); Twist Bioscience DNA library preparation (whole genome, whole exome and UNCseq^TM panels); NEBNext Enzymatic Methylation DNA library preparation (whole genome, methylome); Illumina RNA library preparation (TruSeq Stranded mRNA and TruSeq Stranded Total RNA); Twist Bioscience RNA library preparation (RNA Exome); NanoString GeoMx library preps; iSeq library QC; NextSeq2000 sequencing; NovaSeqX sequencing; NanoString CosMx assays.

Milestones

TGL was a library preparation site for the UNCseq clinical sequencing initiative (LCCC1108/NCT01457196) and The Cancer Genome Atlas (TCGA). The facility has supported >40 clinical trials, >20,000 nucleic acid extractions, >20,000 NGS library preparations, and >20,000 sequenced libraries.

Equipment

• Sample and Reagent Storage – two 4°C refrigerators, five -20°C freezers, and three -80°C freezers that are continuously tracked by Minus80 Monitoring
• Sample Processing – two biological fume hoodsTissue Homogenization – one Omni International Bead Ruptor Elite
• Cell Counters – one Invitrogen Countess II, one Invitrogen Countess 3 FL
• Nucleic Acid Extraction – two KingFisher Flex Systems
• Nucleic Acid QC – three Qubit Flex, two Agilent TapeStations
• Library Preparation – one 10x Genomics Chromium iX Controller, two Hamilton NGS STAR liquid handlers
• Next-Generation Sequencing – one Illumina iSeq, one Illumina NextSeq2000, one Illumina NovaSeqX
• Spatial Multiomics Single-Cell Imager – one NanoString CosMx

Website

https://unclineberger.org/tgl/

Contacts