High Throughput Sequencing Facility

Overview of Services

Welcome to the High Throughput Sequencing Facility at The University of North Carolina.  At the HTSF, our goal is to to enrich medicine and biological research with our state-of-the-art technology.  On this site, you can request DNA sequencing services using Oxford Nanopore Technologies or optical mapping services using the Bionano Saphyr. 

• Oxford Nanopore Technologies:  Nanopore sequencing can read any length of DNA/RNA, from short to ultra-long. It is useful for applications such as de novo assembly, scaffolding and finishing, bridging repetitive regions, structural variation, SNVs and phasing, targeted sequencing, RNA analysis, metagenomics, and epigenetics.  A single flow cell can produce reads >100 Kb and provide up to 10 Gb of sequencing data. 

• Bionano Saphyr:  Optical mapping can detect large-scale structural variations ranging from 500 bp to megabase pair lengths.  It is useful for applications such as de novo assembly of genomes, structural variation in genetic diseases and cancers, Cytogenomics, and scaffolding of sequencing data.  One Saphyr flow cell can produce up to 1300 Gb of data.

Leadership

Gregory W. Bowen |  Director, Integrated Genomics Cores | 919-962-4439 | greg_bowen@med.unc.edu

Piotr A. Mieczkowski | Technical Director of HTSF | 919-962-4471 | Piotr_Mieczkowski@med.unc.edu

Corbin Jones | Faculty Director of HTSF | 919-962-4443 | cdjones@email.unc.edu

Location and hours of operation

Location	Hours
1153 Genome Sciences Bldg 250 Bell Tower Drive Chapel Hill, NC 27514	Monday - Friday     10am - 4pm

Links and Resources

1. HTSF website
2. Oxford Nanopore website
3. Bionano Genomics website

Contacts

Name	Role	Phone	Email	Location
Piotr A Mieczkowski	Technical Director of HTSF	919-962-4471	Piotr_Mieczkowski@med.unc.edu	4256 Genome Sciences Building
Tara Skelly	Research Specialist	919-966-9180	tskelly@email.unc.edu	4244 Genome Sciences Building

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