Overview of Services
Well-differentiated human airway epithelial cell cultures are instrumental for studying basic and applied aspects of cell biology and physiology relevant to cystic fibrosis (CF) and its treatment. The Marsico Lung Institute (MLI) Tissue Procurement and Cell Culture Core (Core) was established to provide standardized cell cultures for CF research. The Core serves as a central source of normal, CF and disease control cells, tissues and fluids for a wide array of uses. The Core has prepared cells from approximately 8,000 human tissue specimens over the past 35 years, producing over 39 x 109 viable primary human airway epithelial cells over the past four years. Dr. Scott Randell, Core Director, and staff have extensive expertise in all aspects of tissue procurement and airway epithelial cell culture. An ever-increasing need for cells and services has broadened Core functions to meet the growing research demands to study CF and other lung diseases.
Custom Media
BEGM MEDIUM: Bronchial epithelial growth media for expansion of cells on plastic
ALI MEDIUM: Air-liquid interface media for differentiation of cells on porous supports
VALI MEDIUM: Vertex-ALI for differentiation of cells on porous supports (contains Ultroser G)-Please inquire about the availablity of this product prior to ordering
Human Bronchial Epithelial Cells (Fresh Cells or Frozen Cryovials at passage 1)
Normal Donors: Donor history shows no prior chronic lung disease. Smoker or non-smoker cells available.
Diseased Donors: Donor history shows prior chronic lung disease (COPD, Asthma).
Cystic Fibrosis Donors: Homozygous severe CFTR mutation - (F508del/F508del).
Cystic Fibrosis Donors: Heterozygous F508del/second severe CFTR mutation - (F508del/Other).
Cystic Fibrosis Donors: Non F508del CFTR mutations - (Other CF/Other CF).
Other Human Lung Cells (Fresh Cells or Frozen Cryovials at early passage)
Well-Differentiated Human Cell Cultures
Normal/diseased passage two or three human primary lung epithelial cells, cultured in ALI medium on collagen coated porous membranes
Age of maturity ~ 4 weeks in culture
Non-smoker or smoker cells available
Training
The MLI Tissue Core will provide training on basic Core techniques and protocols
MTA Handling Fee
Cost of supplies used to generate shipments of MTA specimens requested
Labor
Cost for MLI Tissue Core staff to perform cell culture or other tasks requested by UNC CF investigators
All human cells test negative for mycoplasma, bacteria, yeast and fungi.
Leadership
Scott H. Randell, PhD | Core Director
Leslie Fulcher / Core Manager / 919-966-7055 / leslie_fulcher@med.unc.edu
Location and Hours of Operation
Location
Hours
125 Mason Farm Rd
Marsico Hall #1109A
Chapel Hill, NC 27599
Monday - Friday
8 am - 5 pm
Links and Resources
Tissue Procurement and Cell Culture Core
Search available services:
View: by category
alphabetically
Name
Description
Price
Basic MLI Core training
Provide training on thawing, counting and seeding primary cells
Making custom media
Coating plastic dishes for expansion of primary cells
Coating porous supports
Cell maintenance on plastic and porous supports
Passing and freezing primary cells
External Academic
$150.00
each
Cystic Fibrosis Donors (UNC Rate) - Homozygous severe CFTR mutations -(F508del/F508del)
Passage 2, Cystic Fibrosis bronchial epithelial cells
Cells expanded using CRC methods
Homozygous severe CFTR mutation - (F508del/F508del)
Price of cells/million
Cells test negative for mycoplasma, bacteria, yeast, and fungi.
Inquire
Diseased HBE Cells (Corporate Rate)
Passage 1, diseased bronchical epithelial cells
Price of cells/million
Cells test negative for mycoplasma, bacteria, yeast, and fungi.
Inquire
Normal Nasal Epithelial Cells (UNC Rate)
Nasal epithelial cells from normal donors
Frozen vials at passage 1
Cost of cells/million
Check availability
Inquire
Well-Differentiated Human Cystic Fibrosis Cell Cultures - 12 mm inserts (External Academic)
Cystic Fibrosis passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 12 mm porous membranes .
Age of cultures, ~ four weeks.
External Academic
$185.00
each
Well-Differentiated Human Cystic Fibrosis Cell Cultures - 12 mm inserts (UNC Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 12 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
Internal UNC-CH
$161.00
each
Well-Differentiated Human Cystic Fibrosis Cell Cultures - 6.5mm inserts (Corporate Rate)
Cystic Fibrosis passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 6.5 mm porous membrane .
Age of cultures, ~ four weeks.
Inquire
Well-Differentiated Human Normal Cell Cultures-12mm inserts (UNC Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 12 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
Inquire
Well-Differentiated Human Normal Cell Cultures- 6.5mm inserts (Corporate Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 6.5 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
External
$70.00
each
Well-Differentiated Human Normal Cell Cultures-6.5 mm inserts in Commerical Media (Corporate Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in Commercial media.
Seeded on collagen coated 6.5 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
External
$86.50
each
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►
Human Bronchial Epithelial Cells (HBE) (1)
Name
Description
Price
Diseased HBE Cells (Corporate Rate)
Passage 1, diseased bronchical epithelial cells
Price of cells/million
Cells test negative for mycoplasma, bacteria, yeast, and fungi.
Inquire
▼
►
Well-Differentiated Human Cell Cultures (6)
Name
Description
Price
Well-Differentiated Human Normal Cell Cultures-6.5 mm inserts in Commerical Media (Corporate Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in Commercial media.
Seeded on collagen coated 6.5 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
External
$86.50
each
Well-Differentiated Human Normal Cell Cultures-12mm inserts (UNC Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 12 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
Inquire
Well-Differentiated Human Normal Cell Cultures- 6.5mm inserts (Corporate Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 6.5 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
External
$70.00
each
Well-Differentiated Human Cystic Fibrosis Cell Cultures - 12 mm inserts (UNC Rate)
Normal passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 12 mm porous membrane .
Age of cultures, ~ four weeks.
Non-smoker or smoker cells available.
Internal UNC-CH
$161.00
each
Well-Differentiated Human Cystic Fibrosis Cell Cultures - 12 mm inserts (External Academic)
Cystic Fibrosis passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 12 mm porous membranes .
Age of cultures, ~ four weeks.
External Academic
$185.00
each
Well-Differentiated Human Cystic Fibrosis Cell Cultures - 6.5mm inserts (Corporate Rate)
Cystic Fibrosis passage 2 (or p3 if requested) epithelial cells cultured in ALI media.
Seeded on collagen coated 6.5 mm porous membrane .
Age of cultures, ~ four weeks.
Inquire
▼
►
Training (1)
Name
Description
Price
Basic MLI Core training
Provide training on thawing, counting and seeding primary cells
Making custom media
Coating plastic dishes for expansion of primary cells
Coating porous supports
Cell maintenance on plastic and porous supports
Passing and freezing primary cells
External Academic
$150.00
each
▼
►
Human Nasal Epithelial Cells (1)
Name
Description
Price
Normal Nasal Epithelial Cells (UNC Rate)
Nasal epithelial cells from normal donors
Frozen vials at passage 1
Cost of cells/million
Check availability
Inquire
▼
►
HBE Primary Cells Expanded using Conditionally Reprogrammed Cells (CRC) Methods (1)
Name
Description
Price
Cystic Fibrosis Donors (UNC Rate) - Homozygous severe CFTR mutations -(F508del/F508del)
Passage 2, Cystic Fibrosis bronchial epithelial cells
Cells expanded using CRC methods
Homozygous severe CFTR mutation - (F508del/F508del)
Price of cells/million
Cells test negative for mycoplasma, bacteria, yeast, and fungi.
Inquire