Welcome!
The UNC Metabolomics and Proteomics (MAP) Core Facility provides comprehensive mass spectrometry-based proteomics and metabolomics services for tissues, cells, biofluids, and other biological samples. We serve the UNC research community as well as external academic, clinical, and industry researchers worldwide. We are committed to educating students and researchers in the fields of proteomics and metabolomics and work closely with investigators from initial experimental design through data analysis, interpretation, and publication. We offer an array of sample preparation and instrumentation services, as well as method development and in-depth consultation to help researchers optimize their experimental design based on their experimental goals. Recognized as a Thermo Fisher Scientific Center of Excellence, our facility is equipped with seven state-of-the-art mass spectrometry systems: Thermo Orbitrap Astral, two Exploris 480s, Q Exactive HF, Fusion Lumos, Exploris 240, and Stellar. In addition to our mass spectrometers, we have a Thermo Ultimate 3000, three Thermo Easy-nLC 1200s, an Evosep One, two Vanquish Neo UHPLCs, three Vanquish Horizon UHPLCs, and two 908 Devices ZipChip capillary electrophoresis systems for upfront separation. An Agilent 1260 Infinity II HPLC is available for offline peptide fractionation. We are also equipped with a Thermo AccelerOme for automated, high-throughput sample preparation and a Seer Proteograph for enrichment of low-abundance proteins from plasma and other biofluids.
We offer a broad range of proteomics services, including large-scale proteomic profiling, plasma profiling with Seer enrichment, global phosphoproteomics, protein identification, protein characterization, post-translational modification (PTM) mapping, chemoproteomics, intact mass analysis, and affinity purification analyses. These services can be performed quantitatively using labeled approaches, including TMT 10-plex, 16-plex, and 18-plex, or label-free approaches using data-dependent acquisition (DDA) or data-independent acquisition (DIA). Comprehensive data analysis is provided using a variety of database search engines and software packages, including Proteome Discoverer, Spectronaut, FragPipe, and Skyline. We also provide bioinformatics and downstream data analysis using tools such as Ingenuity Pathway Analysis (IPA), Gene Ontology, Perseus, and R.
The MAP Core also offers a growing range of metabolomics services, including global metabolite profiling, targeted metabolomics, and custom metabolite panels. Our global metabolite profiling service uses hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution mass spectrometry on a Thermo Orbitrap Exploris 240 to profile polar and semi-polar metabolites in both positive and negative ionization modes. The resulting data include peak areas for known metabolites and unknown features, with compound annotation supported by spectral library matching. Our targeted metabolomics services include quantitative analysis of 18 glycolysis and TCA cycle metabolites using high-resolution LC-MS. We also offer custom targeted metabolomics panels designed around specific pathways, compound classes, or research questions, using both LC-MS and capillary electrophoresis-mass spectrometry (CE-MS) platforms. Assay development and consultation are available to help tailor metabolomics workflows to individual research needs.
Research at the MAP Core is supported by funding from the UNC Nutrition and Obesity Research Center (NORC) and the Lineberger Comprehensive Cancer Center (LCCC).
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UNC MAP Core
101 Mason Farm Rd. CB#7026
111 Glaxo Building
University of North Carolina
Chapel Hill, NC 27599
map_core@med.unc.edu
Please visit our website for more information: https://www.med.unc.edu/proteomics-metabolomics/
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Whitney Stutts, PhD |
Director
|
919-966-8318
|
whitney_stutts@med.unc.edu
|
111 Glaxo Building
|
| Natalie Barker |
Lab Manager
|
natalie_barker@med.unc.edu
|
111 Glaxo Building
|
|
| Angie Mordant |
Project Manager
|
angie_mordant@med.unc.edu
|
111 Glaxo Building
|
|
| Steve Bremmer, PhD |
Proteomics Lead
|
Steven_Bremmer@med.unc.edu
|
