Welcome!
The UNC Metabolomics and Proteomics (MAP) Core facility provides services for the analysis of proteins from tissues, cells or other biological samples. We are available to the UNC community, as well as external customers worldwide. We’re committed to educating students and researchers in the field of proteomics and will work with you from initial experimental design through publication.
We offer an array of sample preparation and instrumentation services, as well as method development and in-depth consultation to help researchers optimize their experimental design based on their experimental goals. The facility is equipped with seven state-of-the-art mass spectrometry systems: Thermo QExactive HF, Thermo Fusion Lumos Tribrid, Two Thermo Exploris480, Thermo Exploris240, Thermo Stellar and Thermo Orbitrap Astral. In addition to the mass spectrometers, we have several LCs include: Thermo Ultimate3000, Thermo Easy nLC 1200s, Vanquish Neo UHPLC, Vanquish Horizon UHPLC, Evosep One, as well as two 908 Devices ZipChip capillary electrophoresis devices for up-front separation. We have an Agilent 1260 Infinity II HPLC for offline peptide fractionation. We are also now equipped with a Thermo AccelerOme for automated high-throughput sample preparation.
We offer several proteomics services including: protein identification, protein characterization, post-translational modification (PTM) mapping / profiling (for example: phosphoproteomics, acetylomics), large-scale proteomic profiling, intact mass analysis, and affinity purification analyses. We can perform these services quantitatively using labeled (TMT10plex/16plex/18plex) or label-free approaches (DDA or DIA). Comprehensive data analysis is provided through a variety of database search engines and software packages including Proteome Discoverer, MaxQuant, Byos, Spectronaut, and Skyline. We can also aid in bioinformatics analysis using Ingenuity Pathway Analysis (IPA), Gene Ontology, Perseus, and R.
We recently started offering metabolomics services as well, including a broad metabolomics panel and custom target panels (including TCA/glycolysis panel). Untargeted metabolomics services coming soon!
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Directions on how to set up and navigate iLab can be found here.
Laura Herring, PhD | Facility Director
Office: 111 Glaxo Building
Phone: 919-966-4681
Email: laura_herring@med.unc.edu
UNC MAP Core
101 Mason Farm Rd. CB#7026
111 Glaxo Building
University of North Carolina
Chapel Hill, NC 27599
Please visit our website for more information: https://www.med.unc.edu/proteomics-metabolomics/
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Laura Herring, PhD |
Facility Director
|
919-966-4681
|
laura_herring@med.unc.edu
|
111 Glaxo Building
|
| Whitney Stutts, PhD |
Associate Director - Metabolomics
|
919-966-8318
|
whitney_stutts@med.unc.edu
|
111 Glaxo Building
|
| Natalie Barker |
Lab Manager
|
natalie_barker@med.unc.edu
|
111 Glaxo Building
|
