UNC core facilities will be closed December 23 through January 2. Please contact specific core facilities or refer to the applicable core site for specific closure information.
For UNC core facilities: Thursday December 19th will be the last day to submit billing events (“Send to UNC”) to ensure they are posted in December.
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11/27/2024
Bring your badge to access the core. Safety has always been the Core’s priority. For more information, see page.
Join our Teams page HERE for upcoming events and interesing papers
Staff is happy to consult with you on:
Initiate Request for Consultation Service. Note consultations are FREE for UNC investigators.
We offer the ability to sort a range of particles in our facility including, but not limited to: lymphocytes, tumor digests, cell lines, bacteria, yeast, single cell deposition into wells, tissue digests, virus-infected cells, beads, etc. We have the capability to sort BSL1 to BSL2+ samples. We do not currently have the capability to sort BSL3 and above items. We use nozzles ranging from 70-100 um. Required reading: Sorting Guides: Practical Cell Sorting and the UNC Flow Cytometry Core Facility Sorting SOP. This request is to isolate a population of cells from a sample for downstream analysis.
**Note: Staff checks submissions daily, however, availability depends on instrument AND staff time. Notice of 1 week is preferred; however, times/dates are not guaranteed. Staff do their best to meet your preferred time/date.
**Note: Responses are expected within 48 hours (about 2 days). Instruments are used by independent users and staff, ALL TIMES GIVEN ARE TENTATIVE until the appointment is made.
If you have questions or would like to speak with staff prior to scheduling a sort appointment, submit a Consultataion Request (free).
We offer acquisition services on all of our analyzer instruments. We have a wide variety of instruments that suit various experimental needs (See our website for the full list and optical configurations). Staff is happy to consult with panel and experimental design to ensure successful analysis as well. This request is to have staff run fully prepared samples and provide you with data files for analysis.
**Note: Staff checks submissions daily, however, availability depends on instrument AND staff time. Notice of 1 week is preferred; however, times/dates are not guaranteed. Staff do their best to meet your preferred time/date.
**Note: Responses are expected within 48 hours (about 2 days). Instruments are used by independent users and staff, ALL TIMES GIVEN ARE TENTATIVE until the appointment is made.
Information on the complete training process is located on our website here.
We offer training on all our analyzers in our facility. Once completed you will have 24/7 independent access to the instrument trained on. This process begins with online modules completed at your own pace followed by instrument-specific training with staff. Note, instrument training may take several sessions depending on staff's judgement of user ability. Independent access to the instrument is done at staff discretion.
For information on available analyzers and their optical configurations refer to our website. For help choosing an instrument that best fits your needs, you can refer to this chart or email staff at uncflowcyto@med.unc.edu
We offer training to independently sort BSL-1 non-biohazardous material on the Melody instrument. Those who wish to be trained must complete the necessary online module is Work-flow (Cytometry Select 1- Cell Sorting along with the Flow Cytometry Essentials course; to get access to Work-Flow see Introductory Training ) as well as meet the eligibility requirements outline on our website here. Note, instrument training may take several sessions depending on staff's judgement of user ability.
To Request Cell Sorting Training: Fill out the 'Instrument Training Form (refresher or addional instruments)' form under 'Request Services' Tab. If you have not been trained on any other instruments in the core, see "Introductory Training" above.
Independent access to the instrument is done at staff discretion. Once training has been completed, independent users may operate the instrument at any time during Core hours given availability on the schedule. If afterhours access is requested, additional training is required.
We provide site licenses for the FlowJo portal for our users. Users are charged annually. Note, we will charge the account to the PI you were with when the license was made, not necessarily the PI you are with when we send the bill out. If you change labs, let staff know.
We offer two different licenses:
To request a site license, submit a Data Analysis Software License Request . FlowJo offers a 30-day free trial, if you are interested in trying the software out prior to buying the license.
We provide a license to access FCSE7 to our users. Users are charged annually. Note, we will charge the account to the PI you were with when the license was made, not necessarily the PI you are with when we send the bill out. If you change labs, let staff know.
To request a license, submit a Data Analysis Software License Request .Email uncflowstaff@med.unc.edu with questions. FCES7 is available for a 30-day free trial here.
Name | Role | Phone | Location | |
---|---|---|---|---|
Ramiro Diz |
Director
|
919-966-7812
|
ramiro_diz@med.unc.edu
|
B025 Marsico Hall
|
Janet Dow |
Research Specialist
|
919-966-1530
|
janet_dow@med.unc.edu
|
B023 Marsico Hall
|
Ayrianna Woody |
Research Specialist
|
919-966-1530
|
ayrianna@med.unc.edu
|
B023 Marsico Hall
|
Flow Facility Inbox |
919-966-1530
|
uncflowcyto@med.unc.edu
|
B023 Marsico Hall
|
|
Robert (Bob) Immormino |
Research Specalist
|
919-966-1530
|
rimmormi@email.unc.edu
|
B023 Marsico Hall
|
Service Rate List |
► CellRaft (3) | |||
Name | Description | Price | |
---|---|---|---|
Cell Microsystems CellRaft, 100 um quad | Inquire | ||
Cell Microsystems CellRaft, 200 um quad | Inquire | ||
Cell Microsystems CellRaft, 3D, 500 um | (view additional details) | Inquire | |
► Equipment Setup Charges (1) | |||
Name | Description | Price | |
Sorting Setup |
Internal UNC-CH
$136.00
each
External $357.50 each |
||
► Technical Assistance & Consultation (2) | |||
Name | Description | Price | |
Consultation |
External
$507.50
each
Internal UNC-CH $0.00 each |
||
Staff Time (per hour) | (view additional details) |
External
$507.50
/hr
Internal UNC-CH $70.00 /hr |
|
► Training (2) | |||
Name | Description | Price | |
Introductory Training |
External
$85.00
each
|
||
Instrument Training |
Internal UNC-CH
$151.00
each
External $1,752.50 each |