UNC Flow Cytometry Core Facility

Facility Website 

Updates:

02/14/2022 UPDATE: The Flow Core is open. By using the Core, investigators agree to abide by the safety protocols outlined in the Reopening SOP. Bring your badge to access the core. Safety has always been the Core’s priority. For more information, see page

 

Services

Staff-assisted Cell Sorting Requests 

We offer the ability to sort a range of particles in our facility including, but not limited to: lymphocytes, tumor digests, cell lines, bacteria, yeast, single cell deposition into wells, tissue digests, virus-infected cells, beads, etc. We have the capability to sort BSL1 to BSL2+ samples. We do not currently have the capability to sort BSL3 and above items. We use nozzles ranging from 70-100 um.  

To request a sorting appointment please send an email to uncflowcyto@med.unc.edu with the subject line 'Sorting Request'. Please include the following information: 

  1. Sample/Cell type (include species of origin, primary v cultured, etc) 
  2. How many samples to be sorted. If known, the percentage of your desired population 
  3. Fluorescence panel (include viability dye being used and any fluorescent proteins present in cells) 
  4. Number of cells/sample you plan to BRING. Note this is different than expected number to get BACK.    

Once a date and time is agreed, you will submit a 'Sorting Request' (Request Services> Sorting Request) where you will provide detailed information. Fill the form out accurately and completely. Failure to do so may result in sub-optimal results or rescheduling of the appointment. 

Staff-assisted Acquistion Requests 

We offer acquisition services on all of our analyzer instruments. We have a wide variety of instruments that suit various experimental needs (See our website for the full list and optical configurations). Staff is happy to consult with panel and experimental design to ensure successful analysis as well. For prices of our instrumentation, navigate to the 'Schedule Equipment' Tab> Open 'Analysis Cytometers' Category> Select 'Pricing' to view your rate. Note, this does not include staff time (charged at an hourly rate).   

To request an acquisition appointment, send an email to uncflowcyto@med.unc.edu with the subject line 'Acquistion Request'. Please include the following information: 

  1. Sample/Cell Type 
  2. Total number of samples to be analyzed. Do you plan on bringing samples in tubes or a plate format? 
  3. Fluorescent panel (include any fluorescent proteins and viability dyes) 
  4. Preferred instrument. If you do not have one, staff will consult with you on what is most appropriate based on the information provided 

Once a date and time is agreed, you will submit an 'Acquisition Request' (Request Services> Acquisition Request) where you will provide detailed information. Fill the form out accurately and completely. Failure to do so may result in sub-optimal results or rescheduling of the appointment. 

Training Requests 

Information on the complete training process is located on our website here 

Introductory Training 

  • New customers must complete Work-Flow online class in order to request Instrument-Specific Training 
  • Navigate to the 'Request Services' Tab> Initiate Request for Introductory Training Registration to sign up and you will be contacted within 2 business days with information to proceed. 
  • Once you have completed the modules, please review the UNC-specific module for Core specific information and policies. 

Analyzer Training Requests 

We offer training on all our analyzers in our facility. Once completed you will have 24/7 independent access to the instrument trained on. This process begins with online modules completed at your own pace followed by instrument-specific training with staff. Note, instrument training may take several sessions depending on staff's judgement of user ability. Independent access to the instrument is done at staff discretion.  

  • After completing the Introductory Training, fill out the Instrument Training Request Form to schedule your Instrument-Specific Training. Please allow 2 business days for staff to respond.  
  • Initial instrument training is conducted with core-provided beads. Within 2 weeks, you are required to run an acquisition appointment with you cells under direction of your trainer. Plan accordingly as staff schedules are often busy.  
  • If you are already trained on an instrument in the core and would like training on a different instrument, contact staff at uncflowcyto@med.unc.edu 

For information on available analyzers and their optical configurations refer to our website. For help choosing an instrument that best fits your needs, you can refer to this chart or email staff at uncflowcyto@med.unc.edu  

 

Independent Cell Sorting Training Requests 

 

We offer training to independently sort BSL-1 non-biohazardous material on the FACSAria III instrument. Those who wish to be trained must complete the necessary online module is Work-flow (Cytometry Select 1- Cell Sorting along with the Flow Cytometry Essentials course; to get access to Work-Flow see 'Training Requests') as well as meet the eligibility requirements outline on our website here 

Once training has been completed, independent users may operate the instrument at any time they like given availability on the schedule. Optimal times for use are after business hours of the Facility. Note, instrument training may take several sessions depending on staff's judgement of user ability. Independent access to the instrument is done at staff discretion.  

 

Consultation Requests 

Staff is happy to consult with you on: 

  • Panel Design 
  • Experimental Design 
  • New Techniques 
  • Troubleshooting 
  • Best Practices 

To schedule a meeting, contact us at uncflowcyto@med.unc.edu  with the Subject line 'Consultation Request'. Provide us with a brief description of your experiment aims and how you would like Flow Cytometry to help with that.  

FlowJo Site License 

We provide site licenses for the FlowJo portal for our users. Users are charged annually. Note, we will charge the account to the PI you were with when the license was made, not necessarily the PI you are with when we send the bill out. If you change labs, let staff know. 

We offer two different licenses: 

  1.  Individual: Attached to an individual email. Can be used up to 4 separate PCs (only 4 per year). These computers cannot be used at the same time and should be used by one individual. 
  2. Shared PC:  Attached to a PC address (Single PC), typically a lab computer. Any user (with a [free] FlowJo account ) can then use the software but only on that PC. 

To request a site license, email staff at uncflowcyto@med.unc.edu with the type of license you want. FlowJo offers a 30-day free trial, if you are interested in trying the software out prior to buying the license.  

 

FCS Express 7 License 

We provide a license to access FCSE7 to our users. Users are charged annually. Note, we will charge the account to the PI you were with when the license was made, not necessarily the PI you are with when we send the bill out. If you change labs, let staff know. 

To request a license, view the instructions on our website. Email uncflowstaff@med.unc.edu with questions. FCES7 is available for a 30-day free trial here.  

Contacts

Name Role Phone Email Location
Ramiro Diz
Director
 
919-966-7812
 
ramiro_diz@med.unc.edu
 
B025 Marsico Hall
 
Janet Dow
Research Specialist
 
919-966-1530
 
janet_dow@med.unc.edu
 
B023 Marsico Hall
 
Ayrianna Woody
Research Specialist
 
919-966-1530
 
ayrianna@med.unc.edu
 
B023 Marsico Hall
 
Roman Bandy
Research Specialist
 
919-966-1530
 
rbandy@email.unc.edu
 
B023 Marsico Hall
 
Flow Facility Inbox

 
919-966-1530
 
uncflowcyto@med.unc.edu
 
B023 Marsico Hall
 

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